发明名称 |
Unconventional nucleotide substitution in temperature selective RT-PCR |
摘要 |
Methods are provided for distinguishing between RNA and DNA templates in an amplification reaction. In a preferred embodiment of the invention, the amplification reaction is a PCR and the reaction is catalyzed by a thermostable DNA polymerase or both reverse transcription and amplification of a target RNA. The invention particularly relates to selective amplification of RNA in the presence of homologous DNA, for example, HIV nucleic acids.
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申请公布号 |
US5618703(A) |
申请公布日期 |
1997.04.08 |
申请号 |
US19940199509 |
申请日期 |
1994.02.22 |
申请人 |
HOFFMANN-LA ROCHE INC. |
发明人 |
GELFAND, DAVID H.;MYERS, THOMAS W. |
分类号 |
C12N9/12;C12N15/70;C12P19/34;C12Q1/68;C12Q1/70;(IPC1-7):C12Q1/68 |
主分类号 |
C12N9/12 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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