发明名称 |
ARF-p19, A NOVEL REGULATOR OF THE MAMMALIAN CELL CYCLE |
摘要 |
The INK4A (MTS1, CDKN2) gene encodes a specific inhibitor (InK4a-p16) of the cyclin D-dependent kinases CDK4 and CDK6. InK4a-p16 can block these kinase from phosphorylating the retinoblastoma protein (pRb), preventing exit from the G1 phase of the cell cycle. Deletions and mutations involving the gene encoding InK4a-p16, INK4A, occur frequently in cancer cells, implying that INK4a-p16, like pRb, suppresses tumor formulation. However, a completely unrelated protein (ARF-p19) arises in major part from an alternative reading frame of the mouse INK4A gene. Expression of an ARF-p19 cDNA (SEQ ID NO:1) in rodent fibroblasts induces both G1 and G2 phase arrest. Economical reutilization of protein coding sequences in this manner is without precedent in mammalian genomes, and the unitary inheritance of INK4a-p16 and ARF-p19 may reflect a dual requirement for both proteins in cell cycle control.
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申请公布号 |
WO9712060(A1) |
申请公布日期 |
1997.04.03 |
申请号 |
WO1996US15312 |
申请日期 |
1996.09.25 |
申请人 |
ST. JUDE CHILDREN'S RESEARCH HOSPITAL |
发明人 |
SHERR, CHARLES, J.;QUELLE, DAWN, E. |
分类号 |
A01K67/027;A61K31/00;A61K38/00;A61K39/395;A61K48/00;A61P35/00;A61P43/00;C07K7/08;C07K14/47;C07K16/18;C07K19/00;C12N1/21;C12N5/10;C12N15/00;C12N15/09;C12P21/02;C12Q1/68;C12R1/91;G01N33/53;(IPC1-7):C12Q1/68;C12P21/06;C12N5/00;C07K1/00;C07K16/00;C07H21/04 |
主分类号 |
A01K67/027 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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