| 摘要 |
Lantibiotics, such as nisin and subtilin, are peptides that are substantially modified post-translationally, such as by dehydration of serines and threonines and by formation of thioether crosslinkages. Lantibiotics are synthesized with a leader region that directs proper processing; this leader is specific both for the synthesizing organism and for the mature sequence. Chimeric lantibiotics were synthesized that contained the subtilin leader region and either a subtilin-nisin fusion or a nisin-subtilin fusion for the mature region. The nisin-subtilin chimera was efficiently processed into a functional lantibiotic, but the subtilin-nisin fusion produced a heterogeneous mixture of products, none of which had the expected properties of a correctly process polypeptide. This mixture contained a minor component with a specific activity exceeding that of nisin itself.
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