发明名称 |
Process for separating alpha-1-proteinase inhibitor from COHN IV1+1V4 paste |
摘要 |
The present invention is directed to a process for purifying alpha-1-PI. The process comprises providing an impure protein fraction which comprises alpha-1-PI. The impure protein fraction is suspended in an aqueous solution at pH 6. Insoluble proteins are recovered and resuspended in aqueous solution at pH 8.5. PEG is added to precipitate alpha -2 proteins. To the PEG supernatant precipitation, which comprises alpha-1-PI, is added ZnCl2 to precipitate crude alpha-1-PI. The crude alpha-1-PI is resolubilized and applied to an anion-exchange medium. A fraction comprising alpha-1-PI is recovered from the anion-exchange medium. Alpha-1-PI purified by the process has a specific activity about 1.0 units/OD280.
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申请公布号 |
US5616693(A) |
申请公布日期 |
1997.04.01 |
申请号 |
US19960673064 |
申请日期 |
1996.07.01 |
申请人 |
ALPHA THERAPEUTIC CORPORATION |
发明人 |
HWANG, DUK S.;NARIO, EVELYN;LEPE, MARK;LUZ, LYNDON;ITO, HIROKAZU;TAKECHI, KAZUO |
分类号 |
C12N9/64;C07K1/14;C07K14/775;C07K14/81;(IPC1-7):C07K14/81 |
主分类号 |
C12N9/64 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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