发明名称 MEASUREMENT OF ENZYMIC ACTIVITY AND REAGENT
摘要 PROBLEM TO BE SOLVED: To avoid effects of hemoglobin in a specimen such as a blood serum and accurately measure the enzymic activities such asγ-glutamyltransferase (γ-GTP) by mixing a reagent for measuring the enzymic activities containing a sulfoxy inorganic salt-based reducing agent with the specimen and then measuring a change in absorbance of the mixture. SOLUTION: A disulfite, a hydrogensulfite, a sulfite or a dithionite, etc., as a sulfoxy inorganic salt type reducing agent is added to a reagent for measuring the enzymic activities to carry out the measurement in the method for measuring the activities of a specific enzyme in a specimen comprising mixing a reagent for measuring the enzymic activities such as aγ-glutamyltransferase (γ-GTP) represented by the formula (R is H or COOH), etc., with the specimen such as a blood serum and then measuring a change in absorbance of the resultant mixture in a wavelength region of 400-450nm. Thereby, effects of hemoglobin in the specimen are avoided to suppress a produced error in measured values and more accurately measure the objective enzymic activities.
申请公布号 JPH0984598(A) 申请公布日期 1997.03.31
申请号 JP19950267956 申请日期 1995.09.22
申请人 NITTO BOSEKI CO LTD 发明人 TANAKA HIROTOSHI;KATAYAMA KATSUHIRO
分类号 C12Q1/48;C12Q1/00;G01N33/50;(IPC1-7):C12Q1/48 主分类号 C12Q1/48
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