发明名称 |
Recombinant DNA expression vectors and DNA compounds that encode deacetoxycephalosporin C synthetase and deacetylcephalosporin C synthetase |
摘要 |
<p>The present invention relates to DNA compounds and expression vectors that encode deacetoxycephalosporin C synthetase (DAOCS) and deacetylcephalosporin C synthetase (DACS) activities. The compounds can be used to construct recombinant DNA expression vectors for a wide variety of host cells, including E. coli, Penicillium, and Cephalosporium. The invention also relates to the regulatory elements of the Cephalosporium acremonium DACS/DAOCS gene. A method for selecting Penicillium transformants, including those able to synthesize cephalosporin antibiotic due to the presence of the DACS/DAOCS-encoding DNA, is also provided. This transformation system utilizes an acetamidase gene for purposes of selecting transformants.</p> |
申请公布号 |
GR3022094(T3) |
申请公布日期 |
1997.03.31 |
申请号 |
GR19960403542T |
申请日期 |
1996.12.18 |
申请人 |
ELI LILLY AND COMPANY |
发明人 |
INGOLIA, THOMAS DOMINICK;QUEENER, STEPHEN WYATT;SAMSON, SUELLEN MARY;SKATRUD, PAUL LUTHER |
分类号 |
C12N9/00;C07D501/02;C12N1/14;C12N1/20;C12N9/02;C12N15/00;C12N15/09;C12N15/13;C12N15/52;C12N15/70;C12N15/80;C12P35/06;C12R1/19;C12R1/75;C12R1/82;(IPC1-7):C12N15/00 |
主分类号 |
C12N9/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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