| 摘要 |
PROBLEM TO BE SOLVED: To obtain the subject new animal cell having strengthened transglycosylase activity and capable of producing a glycoprotein such as erythropoietin having a modified structure of sugar chain and expectable for the extension of clearance time from blood by introducing a transglycosylase gene. SOLUTION: A new animal cell derived from the strain CHO-K1, which has a transglycosylase activity strengthened by introducing a gene of a transglycosylase (e.g. N-acetylglucosaminyl transferase V). Culturing of the cell can produce various glycoproteins which are useful for a therapeutic treatment or an industrial purpose, such as erythropoietin which has a modified structure and whose clearance from blood is expected to be extended. The animal cell is obtained by synthesizing an oligonucleotide probe based on a partial amino acid sequence of a transglycosylase of human N-acetylglucosaminyl transferase V, etc., searching a cDNA library of a human fetus liver using the obtained probe, selecting a gene of the transglycosylase, integrating the gene into a vector and introducing the vector into an animal cell. |
