| 摘要 |
<p>According to the present invention, a purified mannanase enzyme obtainable from Bacillus amyloliquefaciens is provided. Preferably, the mannanase of the invention has a molecular weight of about 35,000 when analyzed with the SDS-PAGE method and 20,000 when measured by gel filtration, an isoelectric point (pl) of about 5.2-5.6, a pH optimum of about 4.8-5.2 and a half-life at 80 °C of about 45 seconds. In another embodiment of the invention, a method is provided for the preparation of a purified mannanase derived from Bacillus amyloliquefaciens.</p> |
