| 摘要 |
A method of determining whether or not a particular base is present at a specified position in a nucleic acid sequence. The method comprises the steps of (a) providing a single stranded sample of the sequence to be investigated (the "sample sequence") in immobilised form on a solid support, (b) hybridising a primer to said sample sequence such that the base at the 3' end of the primer is hybridised to that base in the sample sequence which is immediately adjacent to the specified position on the 3' side thereof, (c) treating the primer under extension conditions with a labelled moiety capable of providing an extension unit of the primer at a position corresponding to said specified position in the sample sequence if said particular base is present at that position, the extension conditions being such that any extension of the primer does not continue beyond said specified position of the sample sequence otherwise than by further extension with labelled moiety, (d) washing the support to remove unreacted labelled moiety and other reagents, and (e) testing for incorporation of the labelled moiety in the primer. |
