NUCLEIC ACID REACTIONS

摘要

A method of performing a cycling reaction between RNaseH and a duplex nucleic acid molecule formed from a first (e.g., a probe) and a second (e.g., a target) single strand nucleic acid molecule comprising (1) forming a reaction mixture comprising a plurality of the first single strand molecules, at least one of the second single strand molecules, a single strand binding ligand, and RNaseH, the chemical potential of the single strand binding ligand and the chemical potential of the RNaseH being such that the following cycle of events can occur under isothermal conditions, (a) the formation of a first duplex between an initial first strand and the second strand, (b) a reaction between the RNaseH and the first formed duplex, (c) the dissolution of the first formed duplex, (d) the formation of a second duplex between a subsequent first single strand and the second strand, and (e) a reaction between the RNaseH and the second formed duplex; and (2) allowing at least, wherein n is at least 1, 2, 5, 10, 25, 50, 100, 500, 10<3>, 10<4>, 10<5>, or 10<6>, of the above described cycles to occur.