MUTANTI STABILI DELLA D-N- -CARBAMILASI

摘要

The present invention relates to mutants of D-N- alpha -carbamylase wherein at least one of the cysteines in position 243, 250 and 279 of the amino acid sequence of the wild type enzyme is substituted with a different residue selected from natural amino acids; a recombinant plasmid comprising a nucleotidic sequence which encodes for at least one of the mutants of D-N- alpha -carbamylase, host microorganisms transformed with said plasmid and a process for the preparation of these mutants by the culture of said microorganisms. The mutants of D-N- alpha -carbamylase have a higher enzymatic stability than that of the wild type enzyme and are particularly useful in the preparation of D- alpha -amino acids providing an improvement in the production yields.