| 摘要 |
Methods for the use of a class of dyes for improved DNA sequencing are provided. A new class of dyes, BODIPY TM fluorophore, has been described recently. The parent heterocyclic molecule of the BODIPY TM fluorophore is a dipyrrometheneboron difluoride compound which is modified to create a broad class of spectrally-discriminating fluorophores. The present invention provides methods for the use of BODIPY TM fluorophore-labeled DNA. BODIPY TM fluorophore have improved spectral characteristics compared to conventional fluorescein and rhodamine dyes. BODIPY TM fluorophore have narrower band width, insensitivity to solvent or pH, and improved photostability, thus, BODIPY TM fluorophores lead to improved DNA sequencing and/or detection in any method where electrophoresis and detection of DNA is required. Additionally, the spectral properties of the BODIPY TM fluorophores are sufficiently similar in wavelength and intensity to be used with conventional equipment known in the art.
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