MOLECULE D'ADN CIRCULAIRE A ORIGINE DE REPLICATION CONDITIONNELLE, LEUR PROCEDE DE PREPARATION ET LEUR UTILISATION EN THERAPIE GENIQUE

摘要

A circular DNA molecule, useful for gene therapy, comprising at least one nucleic acid sequence of interest, characterised in that the region allowing the replication thereof has an origin of replication with a functionality in a host cell that requires the presence of at least one specific protein foreign to said host cell. A method for preparing same, cells incorporating said DNA molecules and uses thereof in gene therapy are also described.