Varianten des humanen rekombinanten Interferon-gamma (rhu-IFN-gamma) mit erhöhter thermischer Stabilität

摘要

The invention concerns variants of recombinant human interferon- gamma (rhu-IFN- gamma ) having increased heat-stability. Proceeding from rhu-IFN- gamma , at least one of the following amino acid pairs is changed into cysteine in the amino acid sequence of the polypeptide monomer: Glu8 and Ser70, Ala18 and His112, Lys81 and Leu121, and Gln49 and Leu96. Additionally, the C-terminal end of the polypeptide can be shortened by between 1 and 10 amino acids. The novel proteins according to the invention have greater heat-stability than the unchanged rhu-IFN- gamma without any loss in biological activity. The invention further concerns the preparation of the oligonucleotides, vectors and micro-organisms necessary for producing the proteins according to the invention. Finally, the invention concerns a process for the preparation of the proteins and uses thereof.