METHOD OF ENZYME IMMOBILIZATION, POLYNUCLEOTIDE, VECTOR, CHIMERIC PROTEIN, LOWER EUCARYOTIC ORGANISMS, METHOD OF ENZYMATIC PROCESS REALIZATION

摘要

FIELD: molecular biology, enzymology. SUBSTANCE: method involves immobilization of enzyme or its functional part on microbe cell wall using recombinant DNA procedure. Enzyme is immobilized by its cross-linking with C-terminal protein part that provides attachment to cell wall. Invention relates to recombinant polynucleotide containing structural gene encoding enzyme, part of gene encoding C-terminal protein part that is able to fix on eucaryotic or procaryotic wall, and signal sequence in addition to chimeric protein encoded by recombinant polynucleotide. Also, invention relates to vector and microorganism containing the indicated polynucleotide. Indicated microorganism can be used for large-scale enzymatic processes realization. EFFECT: improved method of enzyme immobilization.