| 摘要 |
PCT No. PCT/JP92/01498 Sec. 371 Date Aug. 8, 1994 Sec. 102(e) Date Aug. 8, 1994 PCT Filed May 19, 1994 PCT Pub. No. WO93/10256 PCT Pub. Date May 27, 1993Disclosed is a method for producing saccarides of definite chain length, such as glucose, maltose, malto-oligosaccharides and isomalto-oligosaccharides with a high purity. Saccharides such as starch, dextran and cellulose, and hydrolyzates thereof, are subjected to modification of the anomeric carbon at the reducing end of the molecule without modification of the non-reducing end of the molecule. The modification may be oxidation, for example by bromine to produce a carboxylic acid at the anomeric carbon, or amination, for example by phenylhydrazine to produce an osazone or osone of the saccharide. After modification, the modified saccharide can be adsorbed on an ion exchange resin and then repeatedly cleaved with a suitable enzyme, such as beta -amylase, to produce the desired saccharide of definite chain length.
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