| 摘要 |
PROBLEM TO BE SOLVED: To obtain a new gene for production, etc., of enzyme, having a specific base sequence, coding a protein having an aromatic acyl group transfer activity and capable of changing flow color and increasing stability of anthocyanin by acylating an anthocyanin-based dye. SOLUTION: This new gene has a base sequence of base sequence numbers 21 to 1412 expressed by the formula and codes a protein having an aromatic acyl group transfer activity and is capable of changing flower color and increasing stability of anthocyanin by acylating a anthocyanin-based dye and is useful for production, etc., of acyl group transferase by recombinant DNA technique. The gene is obtained by separating mRNA from a petal of Gentiana triflora var.japonica by ordinary method, preparing cDNA library using the mRNA and amplifying the cDNA library by polymerase chain reaction using an oligonucleotide derived from RNA of petal of Gentiana triflora var.japonica as a primer. |
