发明名称 |
Process for producing 7 beta -(4-carboxybutanamido)-cephalosporanic acid acylase (GA) enzyme |
摘要 |
The present invention relates to a process for producing large amounts of 7 beta -(4-carboxybutanamido)-cephalosporanic acid acylase ("GA") enzyme, which process consists in growing, under suitable culture conditions, a recombinant Escherichia coli K12 strain and subsequently extracting the enzyme from the resulting culture. Enzyme production is controlled by a particular expression system which can be induced by corn-steep liquor, an extremely low cost component of the culture medium. The enzyme can be used in the enzymatic preparation of 7-amino-cephalosporanic acid by starting from 7 beta -(4-carboxybutanamido)-cephalosporanic acid.
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申请公布号 |
US5612210(A) |
申请公布日期 |
1997.03.18 |
申请号 |
US19940366457 |
申请日期 |
1994.12.30 |
申请人 |
MINISTERO DELL'UNIVERSITA' E DELLA RICERCA SCIENTIFICA E TECNOLOGICA |
发明人 |
VAN DER GOES, WILHELMUS;BERNARDI, ANTONELLA;BOSETTI, ALDO;CESTI, PIETRO;FRANZOSI, GIULIANA |
分类号 |
C12N15/09;C12N1/21;C12N9/80;C12N9/86;C12N15/00;C12N15/31;C12N15/55;C12N15/70;C12P35/02;C12R1/19;C12R1/38;(IPC1-7):C12N1/21;C12N15/72 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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