ANALYTICAL ELEMENT AND METHOD FOR THE DETERMINATION OF A SPECIFIC BINDING LIGAND USING A VANADIUM BROMOPEROXIDASE AS A SIGNAL-GENERATINGENZYME

摘要

An analytical element can be used to sensitively and rapidly detect a wide variety of specific binding ligands in either a competitive or sandwich assay format. The assays are carried out using a vanadium bromoperoxidase-labeled immunoreactant and a chemiluminescent signal-providing wash composition which comprises a 2,3-dihydro-1,4-phthalazinedione derivative; a halogen, pseudohalogen, halogen-providing source or pseudohalogen-providing source; and a peroxide or a peroxide-generating reagent composition.