| 发明名称 |
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| 摘要 |
PURPOSE:To enable quick measurement of substrate concentration in a biological sample, by using a filter film previously subjected to a microwave discharge plasma processing. CONSTITUTION:A platinum wire with the diameter of about 1mm is buried into an insulating substrate 1 made of vinyl chloride resin to form an electrode system comprising an opposed electrode 2, a measuring electrode 3 and a reference electrode 4. A filter film 4 comprising polycarbonate film with the pore diameter of about 1mum and a porous body 6 comprising an nylon unwoven fabrics is provided to cover the exposed surface of the electrode system. The polycarbonate film of the filter film 5 is previously subjected to a plasma processing for about 2 minutes under the condition of about 1-2torr and about 100ml/min using an oxygen-containing gas such as dry air. The porous body 6 is produced by impregnating a nylon unwoven fabrics with a liquid in which about 100mg of glucose oxidase as oxidation reducing enzyme and about 150mg of potassium ferricyanide as electron acceptor are dissolved into about 1ml of a phosphoric acid buffer solution of about pH5.6 and drying it at the room temperature. The biosensor thus obtained can be used to measure the substrate concentration quickly. |
| 申请公布号 |
JP2590803(B2) |
申请公布日期 |
1997.03.12 |
| 申请号 |
JP19840214561 |
申请日期 |
1984.10.12 |
| 申请人 |
MATSUSHITA ELECTRIC IND CO LTD |
发明人 |
NANKAI SHIRO;KAWAGURI MARIKO;IIJIMA TAKASHI |
| 分类号 |
G01N27/327;C12Q1/00;G01N27/27;G01N27/30 |
主分类号 |
G01N27/327 |
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