DNA probes specific for virulent listeria monocytogenes

摘要

An assay for detecting virulent L. monocytogenes is provided. This assay includes the steps of: contacting the nucleic acids of L. monocytogenes with a probe under conditions permitting hybridization; and detecting any probe that hybridizes to the nucleic acids. The probe used in this method includes a DNA sequence selected from a group consisting of a 0.9 kb HindIII-EcoRI fragment of plasmid pLUCH52, or a part thereof; a 1.1 kb HindIII-EcoRI fragment of plasmid pLUCH51, or a part thereof; and a 1.8 kb HindIII-EcoRI fragment of plasmid pLUCH44, or a part thereof).