STRAIN OF BACTERIUM PHOTOBACTERIUM PHOSPHOREUM - A PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND SPLITTING THE NUCLEOTIDE SEQUENCE 5'-GG(T/G)(AG)CC-3'

摘要

FIELD: molecular biology, genetic engineering, biotechnology. SUBSTANCE: invention proposes new strain Photobacterium phosphoreum (VKPM B-6667) - a producer of restriction endonuclease Pph14 I isolated from sea water after restriction endonucleases screening. Enzyme yield is 10000 U/g wet biomass, specific activity of the end enzyme is 20000 U/ml. Restrictase Pph14 I is an isoschizomer of restrictase HgiH I and can replace the latter in all genetic engineering works. Restrictase Pph14 I is thermostable enzyme that recognizes and splits the nucleotide sequence 5'-GG(T/C)(A/G)CC-3'. EFFECT: new strain indicated above, high yield and activity of enzyme. 10 cl, 1 dwg