发明名称 |
RECOMBINANT PLASMID DNA PHBMD1 CONTAINING THE MODIFIED GENE OF MEDIAN SURFACE PROTEIN OF HUMAN HEPATITIS VIRUS B, - INTERMEDIATE PLASMID FOR RECOMBINANT PLASMID DNA YEP 63/AB CONSTRUCTING, RECOMBINANT PLASMID DNA YEP 63/AB ENCODING SYNTHESIS OF MEDIAN SURFACE DERIVATIVE PROTEIN OF HEPATITIS B VIRUS AND STRAIN OF YEAST SACCHAROMYCES CEREVISIAE - A PRODUCER OF MEDIAN SURFACE DERIVATIVE PROTEIN OF HEPATITIS B VIRUS |
摘要 |
FIELD: molecular biology, genetic engineering, medicine. SUBSTANCE: recombinant plasmid pHBmd1 that is inherited in E. coli cells and carrying the modified gene of median surface protein of human hepatitis B virus (M-protein HBV) and containing deletion in specific site of region preS2 that determines sensitivity of M-protein HBV to trypsin-like proteases is obtained on the basis of the cloned gene encoding M-protein HBV. On the basis of plasmid pHBmd1 recombinant plasmid YEp63/AB is constructed. This plasmid provides synthesis of M-protein HBV derivative in the transformed cells of yeast S. cerevisiae, strain 20B12. The presence of intact promoter of yeast gene encoding glyceraldehyde-3-phosphate dehydrogenase in plasmid YEp63/AB located before initiating codon AUG ensures to get high expression level without induction. Synthesized protein assembles particle normally as the natural 22 nm particle HBsAg. Synthesis level of M-protein derivative is 0.5-1% of total protein level in cell. New strain-producer can be used for industrial production of M-protein HBV derivative and used for diagnostic and immunoprophylaxis aims, for development of antihepatitis vaccines of new generation showing the enhanced protective properties. EFFECT: increased proteolytic stability of M-protein HBV derivative, new strain indicated above. |
申请公布号 |
RU94030507(A) |
申请公布日期 |
1997.03.10 |
申请号 |
RU19940030507 |
申请日期 |
1994.08.22 |
申请人 |
TSENTR "BIOINZHENERIJA" RAN |
发明人 |
BIRAGIJN AR'JAA;SKRJABIN K.G.;EHL'DAROV M.A. |
分类号 |
C12N15/36;A61K39/29 |
主分类号 |
C12N15/36 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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