发明名称 |
METHOD OF SUMULTANEOUSLY DETECTING AMPLIFIED NUCLEIC ACID SEQUENCES AND CELLULAR ANTIGENS IN CELLS |
摘要 |
In one aspect, the present invention provides an in situ process of simultaneously detecting a specific predetermined nucleic acid sequence and a specific predetermined cellular antigen in the same cell. In accordance with that process, the antigen is labeled with a biotin- or DNP-tagged antibody that specifically immunoreacts with the antigen, the specific nucleic acid sequences in the cell are amplified, the amplified nucleic acid sequences are labeled with a fluorescently-tagged nucleic acid probe that specifically hybridizes to the amplified nucleic acid sequences, and the labeled nucleic acid sequences and labeled cellular antigen are detected.
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申请公布号 |
WO9708343(A1) |
申请公布日期 |
1997.03.06 |
申请号 |
WO1996US13936 |
申请日期 |
1996.08.30 |
申请人 |
NORTHWESTERN UNIVERSITY;PATTERSON, BRUCE;GOOLSBY, CHARLES, L., JR. |
发明人 |
PATTERSON, BRUCE;GOOLSBY, CHARLES, L., JR. |
分类号 |
C12Q1/68;C12Q1/70;G01N15/14;G01N33/569;(IPC1-7):C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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