METHODS AND ANTIBODIES FOR DETECTING CREATINE KINASE

摘要

Assays and antibodies are provided for detecting creatine kinase (CK) enyzmes. Total CK isoenzyme may be detected with an antibody specific for an epitope within a conserved region of the M subunit and B subunit of CK isoenzymes as well as to mitochondrial CK. The ratio of CK-MB isoenzyme to total CK isoenzyme may be determined simultaneously using a solid phase assay device having a first reaction zone and a second reaction zone. Antibody specific for total CK isoenzymes is present in the first reaction zone while antibody specific for CK-MB isoenzyme is present in the second reaction zone. A single patient plasma or serum sample may then be reacted with the solid phase to effect the simultaneous determination of the relative amounts of CK isoenzyme.