| 摘要 |
<p>The invention of this disclosure relates to a method for producing composite skin comprising a dermis inoculated directly with mammalian cells. In the method of this invention a dermis is obtained, preferably an acellular dermis of xenogeneic or human origin. The cells used to inoculate the dermis are transferred directly or preselected for properties consistent with progenitor or stem cell. The cells are of xenogeneic or human origin. In the practice of the preferred embodiments of this invention cells inoculated on the dermis are allowed to propagate ex vivo in culture to increase their number. In the preferred embodiment of this invention the ex vivo culturing results in the expansion of a population of cells having progenitor characteristics. The cells used to inoculate the dermis, in the preferred embodiments of this invention are genetically modified ex vivo. In the most preferred method of this invention the dermis to be inoculated is of human origin. In this embodiment the cells are either autologous or allogeneic in origin or a combination of both. The inoculated human dermis of this preferred embodiment can be applied to a human or utilized ex vivo as a laboratory assay.</p> |
