摘要 |
<p>An analytical rotor for performing immunoassays comprising one or more inlet chambers (16) for sample, wash reagents, and labelling reagents. A reaction chamber (28) is disposed radially outwardly from the inlet chambers and connected thereto by low flow resistance flow paths (34). A collection chamber (60) is located radially outwardly from the reaction chamber and connected thereto by a high flow resistance flow path (62). Samples are introduced to the sample inlet chamber by a transfer device, with sample volumes optionally determined by detecting when the sample inlet chamber is filled. Reagents initially introduced to the inlet chambers may be selectively transferred to the reaction chamber by low speed rotation of the rotor. The reaction chamber may then be emptied by high speed rotation of the rotor. In this way, heterogeneous immunoassays requiring sequential contact of reaction zones with sample and different reagents may be performed.</p> |