Recombinant 60kda vegetable panallergen (co-factor-independent phosphoglycerate mutase; e.c. 5.4.2.1.)

摘要

The description relates to a recombinant DNA molecule which codes a polypeptide antigen property of the co-factor-independent phosphoglycerate mutase (E.C. 5.4.2.1.) of birch, mugwort or timothy grass pollen. This allergen in birch pollen is highly preserved on sequence and antigen property in all plants (but not in animal organisms). The amino acid sequence and the most important B and T-cell epitopes of the molecule are derived and demonstrated. The recombinant allergen was expressed in E. coli and binds the IgE serum of patients who are allergic to tree, grass and weed pollens and various foodstuffs. A monoclonal antibody (BIP 3) specifically bonds to said highly conserved protein from all plants tested. The significance of the co-factor-independent phosphoglycerate mutase (E.C. 5.4.2.1.) derives from the fact that it results in the cross-sensitisation of patients. The recombinant molecule and its partial peptides can be used in diagnostic and therapeutic methods based, for example, on antigen-antibody interaction, mediator release or T-cell reactivity.