发明名称 |
PROCESS FOR PRODUCING 2-KETO-L-GULONIC ACID |
摘要 |
An expression vector containing both of a DNA coding for L-sorbose dehydrogenase and a DNA coding for L-sorbosone dehydrogenase; a transformant capable of producing 2-keto-L-gulonic acid (2KLGA) from D-sorbitol in a high yield and prepared by transforming with the above vector a microorganism which produces L-sorbose from D-sorbitol in a high yield and which has no or little activity of decomposing 2KLGA, or such a microorganism which has, in addition, no or little activity of producing L-idonic acid; and a process for producing 2KLGA by culturing the transformant in a medium containing D-sorbitol. The invention process enables 2KLGA useful for producing L-ascorbic acid to be produced readily in a large amount at a good efficiency by single culture. |
申请公布号 |
EP0758679(A1) |
申请公布日期 |
1997.02.19 |
申请号 |
EP19950909983 |
申请日期 |
1995.02.24 |
申请人 |
FUJISAWA PHARMACEUTICAL CO., LTD. |
发明人 |
NIWA, MINEO;SAITO, YOSHIMASA;ISHII, YOSHINORI;YOSHIDA, MASARU;HAYASHI, HIROMI |
分类号 |
C12N9/02;C12N9/04;C12N15/53;C12P7/60 |
主分类号 |
C12N9/02 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|