发明名称 |
Methods and materials for restriction endonuclease applications |
摘要 |
The present invention is directed to materials and methods for the quasi-random and complete fragmentation of DNA using restriction endonuclease reagents capable of cutting DNA at a dinucleotide sequence. The invention is also directed to methods for labeling DNA using template-specific oligonucleotides, for shotgun cloning, for sequencing of DNA, for epitope mapping and for anonymous primer cloning, all using fragments of DNA generated by the method of the present invention.
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申请公布号 |
US5604098(A) |
申请公布日期 |
1997.02.18 |
申请号 |
US19940362741 |
申请日期 |
1994.12.22 |
申请人 |
MOLECULAR BIOLOGY RESOURCES, INC. |
发明人 |
MEAD, DAVID;SWAMINATHAN, NEELA |
分类号 |
C12N9/10;C12N9/22;C12N15/10;C12N15/11;(IPC1-7):C12Q1/68;C12P19/34;C07H21/04;C12N15/00 |
主分类号 |
C12N9/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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