PSEUDOMONAS INFECTION PREVENTING VACCINES USING ATTENUATED PSEUDOMONAS CFCPA 60534

摘要

An attenuated pseudomonasaeruginosa isolate especially CFCPA 60534 is disclosed for formulating vaccine curing or preventing pseudomonas contagion. Pseudomonasaeruginosa isolate is extracted from the infected patient and classified into 7 classes by Fisher-Devilin immunotype; only type 6 germ is selected, repeatedly dosed to experimental animals and acclimatized to yield attenuated pseudomonasaeruginosa isolate CFCPA 60534 (KCCM 10034) which is not toxic in itself and attenuated for the cell wall protein to yield only antigen. The acclimation to yield the attenuated pseudomonasaeruginosa isolate is repeated, commonly 3-7 times, until it shows the desired value of LD50. The desired LD50 value is more than 2.0 x 107 cells. CFCPA 60534 can be used for preparing pseudomonasaeruginosa vaccine by nurturing the germs in various sized fermenters having an optimum environment; yielding many isolates; treating the isolates by a series of processes; yielding cell wall protein; and finally treating them by ordinary vaccine preparation methods.