HELICOBACTER PYLORI-COLONIZED MONGOLIAN GERBIL, PROCESS FOR PREPARATION THEREOF, MEDIUM FOR SEPARATING HELICOBACTER PYLORI, AND METHOD OF SCREENING ANTI-HELICOBACTER PYLORI-ACTIVE SUBSTANCE BY USING THE SAME

摘要

Helicobacter pylori-colonized Mongolian gerbils prepared by oral inoculation of a broth culture obtained by liquid culture of Helicobacter pylori, to the Mongolian gerbils for intragastric colonization of Helicobacter pylori. These Mongolian gerbil models are useful for screening the therapeutic efficacies of drugs against Helicobacter pylori, as well as elucidation of pathology of Helicobacter pylori disease in humans. Moreover, the medium of the present invention for isolating Helicobacter pylori, which is supplemented with vancomycin, trimethoprim, amphotericin B, polymyxin B and 2,3,5-triphenyltetrazolium chloride, enables selective and efficient detection of Helicobacter pylori colonized in Mongolian gerbils which have microorganisms living in stomach. A combined use of the above-mentioned Mongolian gerbil models and the medium for isolating Helicobacter pylori enables screening of an anti-Helicobacter pylori substance at an animal level, thereby enabling accurate prediction of clinical effects of the anti-Helicobacter pylori substance.