Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control
摘要
An instrument for performing highly accurate PCR employing a sample block in microtiter tray format. The sample block has local balance and local symmetry. A three zone film heater controlled by a computer and ramp cooling solenoid valves also controlled by the computer for gating coolant flow through the block controls the block temperature. Constant bias cooling is used for small changes. Sample temperature is calculated instead of measured. A platen deforms plastic caps to apply a minimum acceptable threshold force for seating the tubes and thermally isolates them. A cover isolates the block. The control software includes diagnostics. An install program tests and characterizes the instrument. A new user interface is used. Disposable, multipiece plastic microtiter trays to give individual freedom to sample tubes are taught.
申请公布号
US5602756(A)
申请公布日期
1997.02.11
申请号
US19950569606
申请日期
1995.12.08
申请人
THE PERKIN-ELMER CORPORATION
发明人
ATWOOD, JOHN G.;MOSSA, ALBERT C.;GOVEN, LISA M.;WILLIAMS, FENTON;WOUDENBERG, TIMOTHY M.;MARGULIES, MARCEL;RAGUSA, ROBERT P.;LEATH, RICHARD;MILES, CLIVE