发明名称 |
Method for detecting target nucleic acid in specimen |
摘要 |
An intended nucleic acid is determined in a sample by a method in which at least one of the nucleic acid strands of the intended nucleic acid is subjected to hybridization with a primer and to chain-extension reaction over the primer to form a synthesized nucleic acid which is complementary to and in hybridization with the strand; a copy of the intended nucleic acid is obtained, which copy is (a) the nucleic acid of the double-stranded double produced by the chain-extension reaction, or (b) the synthesized nucleic acid freed from the double-stranded nucleic acid, or (c) a double stranded nucleic acid form from a pair of the synthesized nucleic acids complementary with each other; and it is determined whether the copy is present in the sample thereby to know whether the intended nucleic acid is in present in the sample.
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申请公布号 |
US5601976(A) |
申请公布日期 |
1997.02.11 |
申请号 |
US19920945573 |
申请日期 |
1992.09.16 |
申请人 |
WAKUNAGA SEIYAKU KABUSHIKI KAISHA |
发明人 |
YAMANE, AKIO;OKA, TAKANORI;NAKAGAMI, SATORU;MIYOSHI, KENICHI |
分类号 |
C12Q1/68;C12Q1/70;(IPC1-7):C12Q1/68;C07H21/04;C12P19/34;C12N15/00 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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