发明名称 |
Nuclear localized transcription factor kinase and diagnostic assays related thereto |
摘要 |
New nuclear kinases that are regulated by signal transduction and that participate in phosphorylation cascades which regulate transcription and related methods for regulating transcription. The novel nuclear kinases play a vital role in gene expression, particularly with regard to leukemia in humans. The kinase is (i) substantially exclusively intranuclearly localized; (ii) capable of autophosphorylation; (iii) selectively bindable with antibodies raised against the RING3 portion of GST-RING3; (iv) of a molecular weight of from about 82.5 to about 92.7 kilodaltons; and (v) includes peptide sequences Asp-Ser-Asn Pro-Asp-Glu-Ile-Glu-Ile-Asp-Phe-Glu-Thr-Leu-Lys-Pro-Thr-Thr-Leu (SEQ ID NO: 1) and Ala-Val-His-Glu-Gln-Leu-Ala-Ala-Leu-Ser-Gln-Ala-Pro (SEQ ID NO: 2). The invention features a method of detecting leukemic cells in a biological sample, the method includes measuring the activity of a RING3-related kinase in the sample, an increase in the activity relative to a control being indicative of the presence of leukemic cells. |
申请公布号 |
AU6761696(A) |
申请公布日期 |
1997.02.10 |
申请号 |
AU19960067616 |
申请日期 |
1996.07.10 |
申请人 |
UNIVERSITY OF MASSACHUSETTS MEDICAL CENTER |
发明人 |
GERALD V. DENIS;MICHAEL R GREEN |
分类号 |
C12N9/12;C12Q1/48;G01N33/574 |
主分类号 |
C12N9/12 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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