A multiple well tissue culture plate (10) having a plurality of wells (36) with a semi-permeable membrane (20) closing the bottom of each well (36). The multiple well tissue culture plate (10) is placed in contact with a basal medium (26) contained within a basal medium reservoir (12) to allow for exchange of nutrients and waste products between the basal medium (26) and the culture medium (22) across the semi-permeable membrane (20). The dialyzed multiple well tissue culture plate (10) provides dialysis of each individual well (36) within the multiple well tissue culture plate (10) thus eliminating traditional exchange of culture medium to maintain proper culture conditions.
