发明名称 |
Analysförfarande |
摘要 |
Method for determining the activity of a nucleotide-polymerising enzyme in a sample comprises: (a) capturing the enzyme with a monoclonal antibody (MAb) immobilised on a fixed carrier (the MAb binds the enzyme without significantly affecting its activity); (b) removing impurities and/or disturbance factors; (c) starting nucleotide polymerisation by adding a reaction soln. contg. a primer/template construction and nucleotide substrate; (d) providing reaction conditions to favour permanent association between the enzyme and primer/template prod. complex; (e) washing the nucleotide substrate, primer/template and reaction soln. to remove freshly synthesised polymer; (f) measuring the amt. of nucleotide incorporated with the polymer by standard techniques, and (g) determining enzyme activity. |
申请公布号 |
SE9502765(L) |
申请公布日期 |
1997.02.05 |
申请号 |
SE19950002765 |
申请日期 |
1995.08.04 |
申请人 |
CLAS KAELLANDER;JAN SIMON GRONOWITZ |
发明人 |
KAELLANDER CLAS;GRONOWITZ JAN-SIMON;LENNERSTRAND JOHAN |
分类号 |
C07K16/40;C12Q1/42;C12Q1/68;C12Q1/70;G01N33/573;(IPC1-7):C12Q1/68 |
主分类号 |
C07K16/40 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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