RECOMBINANT DNA METHODS, VECTORS AND HOST CELLS

摘要

The present invention relates to a method for transforming a lymphoid cell line to glutamine independence which comprises: transforming the lymphoid cell line with a vector containing an active glutamine synthetase (GS) gene; growing the transformed cell line on a medium containing glutamine; and continuing the growth of the transformed cell line on a medium in which the glutamine is progressively depleted or on a medium lacking glutamine, and to vectors and host cells for use in the method.