| 摘要 |
The present invention relates to the beta -glucuronidase (GUS) gene fusion system, and to the cloning and characterization of the beta -glucuronidase and glucuronide permease genes of Escherichia coli. It is based on the surprising discovery that gene fusions comprising the beta -glucuronidase gene may be effectively expressed in a wide variety of organisms to produce active beta -glucuronidase enzyme. Because of the abundance and availability of useful substrates for beta -glucuronidase enzyme, GUS gene fusions may serve as a superior reporter gene system as well as an effective means of altering cellular phenotype. In conjunction with recombinant glucuronide permease, which may be used to render host cells permeable to beta -glucuronidase substrates, the GUS gene fusion system offers almost unlimited applications in the fields of plant and animal genetic engineering.
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