Device and a method for the electrophoretic separation of fluid substance mixtures

摘要

A device for the electrophoretic separation of complex fluid substance mixtures comprises a channel system (21, 2, 22) for a carrier medium (C), an injection device (3) for the injection into the carrier medium (C) of a substance mixture (S) to be separated, and a separating path (2) for the separation of the substance mixture (S) in an electric field applied along the separating path (2). Downstream of the injection device (3) for the substance mixture (S) to be separated and at a distance therefrom there is provided a second separating path (4) for the further separation of the substance mixture (S) in an electric field applied along the second separating path (4). The second separating path (4) is inclined at an angle ( alpha ) with respect to the first separating path (2). The region of intersection of the first (2) and second (4) separating paths forms a second injection device (5) for injecting the partially separated substance mixture (S) into a second carrier medium (E). The fluid substance mixture (S) is injected into the carrier medium (C) in the first injection device (3) and is then separated in the electric field that is applied along the first separating path (2). The partially separated substance mixture (S) is then injected into the second carrier medium (E) downstream of the first separating path (2) and further separated into its components in the electric field applied along the second separating path (4).