| 摘要 |
<p>A method for rapidly detecting the presence or absence of a particular nucleic acid sequence at a candidate locus in a target nucleic acid sample comprises the steps of: i) introducing a modified base which is a substrate for a DNA glycosylase into said candidate locus at one or more preselected positions; ii) excising the modified base by means of said DNA glycosylase so as to generate an abasic site; iii) cleaving phosphate linkages at abasic sites generated in step ii); and iv) analysing the cleavage products of step iii) so as to identify in said target nucleic acid sequence the presence or absence of said particular nucleic acid sequence at said candidate locus. The method has particular application for detecting specific mutations in a DNA sample, including the detection of multiple known mutations in DNA. Large throughput of sample can be achieved rapidly and easily.</p> |
