| 摘要 |
PROBLEM TO BE SOLVED: To obtain a new ligand, a glycoprotein having specific molecular weight with the sugar chain representing chondroitintetrasulfuric acid, having serine-glycine recurring unit, capable of activating cytotoxic T-cells via CD44, and useful for the research and prevention of cancer. SOLUTION: This new ligand is for protein CD44 lying on cell surface and has an important role concerning metastasis or lymphocyte homing and useful as e.g. a reagent for chancer research or a cancer preventive, having the following characteristics: a glycoprotein (proteoglycan) about 600,000 in molecular weight; the molecular weight of the protein portion (chore protein) is about 18,000-22,000; the sugar chain represents chondroitintetrasulfuric acid; the amino acid sequence of the protein contains serine-glycine recurring units; capable of activating cytotoxic T-cells via CD44 borne by the T-cells; and capable of binding to the CD44 on a site differing from the binding site for the CD44 and hyaluronic acid. This new glycoprotein is obtained by purifying the supernatant resulted from culturing serglycine-productive cells through an anion exchange resin, gel filtration and a hydroxyapatite column. |
