发明名称 |
Sulfonamide resistance genes and their use |
摘要 |
Plant cells are transformed by: (i) transforming a plant cell whose growth is sensitive to inhibition by a sulfonamide or a salt thereof with a chimaeric gene comprising (a) a plant promoter, (b) a sulfonamide resistance gene having a sequence encoding a transmit peptide fused to the 5'-end of the resistance gene and (c) a plant polyadenylation/terminator sequence; (ii) selecting a transformed plant cell whose growth is resistant to inhibition by a sulfonamide or salt thereof; (iii) optionally, regenerating from the transformed plant cell a genetically transformed plant which exhibit the said resistance; (iv) optionally, obtaining seed from the regenerated plant; and (v) optionally, propagating plants from the seed. The growth of weeds can be controlled at a locus where a transgenic plant obtained as above is being cultivated by applying to the locus an effective amount of a herbicide, such as asulam, which acts by inhibiting dihydroptercate synthase. The sulfonamide resistance gene can be also used as a selectable marker. The sequence of a sulfonamide resistance gene is presented.
|
申请公布号 |
US5597717(A) |
申请公布日期 |
1997.01.28 |
申请号 |
US19950444860 |
申请日期 |
1995.05.19 |
申请人 |
RHONE-POULENC AGROCHIMIE LIMITED |
发明人 |
GUERINEAU, JEAN F.;MULLINEAUX, PHILIP M.;PARNELL, EDGAR W. |
分类号 |
A01H1/00;A01H5/10;C12N9/10;C12N15/31;C12N15/54;C12N15/82;C12N15/84;(IPC1-7):C12N15/82 |
主分类号 |
A01H1/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|