MICROBIAL STRAIN PRODUCTION 6-PHOSPHOGLUCONIC ACID DEHYDROGENASE

摘要

PROBLEM TO BE SOLVED: To obtain the subject new microbial strain producing a 6- phosphogluconic acid dehydrogenase having an activity in a crude enzyme solution smaller in the case of using NADP as the coenzyme than in the case of using NAD as the coenzyme, having high stability and giving an enzyme e.g. for various determination reagents. SOLUTION: This new microbial strain produces 6-phosphogluconic acid dehydrogenase [e.g. Lactobacillus sp. SHO84 (FERM P-15038)]. The activity value of the enzyme in a crude enzyme solution in the case of using nicotinamide adenine dinucleotide phosphate (NADP) as the coenzyme is <=10% of the activity value attained by using nicotinamide adenine dinucleotide (NAD) as the coenzyme. It is useful e.g. for the production of 6-phosphogluconic acid dehydrogenase having high specificity to NAD, exhibiting excellent stability and widely usable for various determination reagents, etc.