| 摘要 |
PURPOSE:To measure a measuring component with good accuracy up to a high-concn. region by installing an immobilized layer of oxidase on an electrode system and bringing a layer which consists of a water soluble mediator deposited in a dry state on a insoluble porous film into tight contact with the surface of said layer. CONSTITUTION:A measuring electrode 2, a counter electrode 3 and an insulating layer 4 are provided on an insulating substrate 1. An aq. soln. of 5% carboxymethyl cellulose is applied on this electrode system and is dried. Furthermore, a glucose oxidase soln. is coated as an enzyme soln. thereon and is dried to produce the enzyme immobilized layer 5. The mediator deposited layer 6 produced by impregnating a phosphate buffer soln. of potassium ferricyanide as the mediator to a porous cellulose film and drying the same is held in pressurized contact with the surface of the enzyme immobilized layer 5 by a holding frame 7. A large amt. of the potassium ferricyanide is thereby supplied into a sample liquid and the migration of the sample reaction liquid between the respective layers is smoothly executed at the time of measurement and, therefore, the objective component is measured with the good accuracy up to the high concn. |
