Method for obtaining protective active and inactive adenylate cyclase from Bordetella parapertussis

摘要

A method for preparing an active and inactive adenylate cyclase from a culture of Bordetella parapertussis having specific reactivity with polyclonal or monoclonal antibodies to adenylate cyclase from Bordetella pertussis is presented. The inactive adenylate cyclase is devoid of calmodulin-activatable adenylate cyclase activity and of affinity for calmodulin. The method comprises culturing a clone of Bordetella parapertussis, homogenizing the culture to produce a homogenate, and isolating the active and inactive adenylate cyclase from the homogenate by urea precipitation. The isolated active and inactive adenylate cyclase may be used to prepare a vaccine for the prevention of Bordetella infection.