发明名称 |
A novel beta1->6 N-acetylglucosaminyltransferase, its acceptor molecule, leukosialin, and a method for cloning proteins having enzymatic activity |
摘要 |
The present invention provides a novel beta 1->6 N-acetylglucosaminyltransferase, which forms core 2 oligosaccharide structures in O-glycans, and a novel acceptor molecule, leukosialin, CD43, for core 2 beta 1->6 N-acetylglucosaminyltransferase activity. The amino acid sequences and nucleic acid sequences encoding these molecules, as well as active fragments thereof, also are disclosed. A method for isolating nucleic acid sequences encoding proteins having enzymatic activity is disclosed, using CHO cells that support replication of plasmid vectors having a polyoma virus origin of replication. A method to obtain a suitable cell line that expresses an acceptor molecule also is disclosed. <IMAGE> |
申请公布号 |
AU7041996(A) |
申请公布日期 |
1997.01.16 |
申请号 |
AU19960070419 |
申请日期 |
1996.10.25 |
申请人 |
LA JOLLA CANCER RESEARCH FOUNDATION |
发明人 |
MINORU FUKUDA;MARTI F. A. BIERHUIZEN |
分类号 |
C12N15/09;C07K14/47;C07K14/705;C07K16/40;C12N1/21;C12N5/10;C12N9/10;C12N15/54;C12N15/64;C12P21/02;C12R1/91 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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