Non-isotopic detection of nucleic acids using a polystyrene support-based sandwich hybridization and compositions useful therefor

摘要

PCT No. PCT/US91/08523 Sec. 371 Date May 13, 1993 Sec. 102(e) Date May 13, 1993 PCT Filed Nov. 14, 1991 PCT Pub. No. WO92/08808 PCT Pub. Date May 29, 1992.Methods for the detection of nucleic acid using sandwich hybridization are provided. The target nucleic acids are captured by hybridization with oligonucleotides covalently attached to polystyrene solid supports to form complexes which are then hybridized to detection oligonucleotides to facilitate the analysis of the target nucleic acid sequences.