| 摘要 |
PROBLEM TO BE SOLVED: To obtain the subject protein, a kind of virus beloging to Granulosis virus, stemmed from virus separated from e.g. Xestia c-nigrum, capable of potentiating infective power of nuclear polyhedrotic virus, thus having controlling effect on butterfly pests in farm products. SOLUTION: This new protein with a molecular weight of about 100Kda is stemmed from a kind of virus belonging to Granulosis virus and isolated from Xestia c-nigrum, Leucania separata, Helotropha leucostigma laevis, Hydroecia amurensis, Apamea secalis or Autographa excelsa, and capable of potentiating infective power of nuclear polyhedrotic virus, therefore being usable for e.g. pest control by its infection on butterfly pests in farm products through potentiating inefective power of the nuclear polyhedrotic virus. This new protein is obtained by the following process: distilled water is added to dead Xestia c-nigrum larvae infected with Granulosis virus followed by grinding the dead larvae and then conducting a filtration and centrifugal separation to isolate the virus, which is then treated with an alkali and subsequently ultracentrifuged, and the resultant supernatant is subjected to chromatography. |
