发明名称 |
DNA sequence-based HLA class I typing method |
摘要 |
The present invention provides a process for determining genotypes in highly polymorphic systems by polymerase chain reaction amplification of cDNA or genomic DNA and direct sequencing polymerase chain reaction products using oligonucleotide primers. More specifically, Class I HLA genotypes can be unambiguously determined in any subject in 16-24 hours by direct sequencing of HLA-A, HLA-B, and HLA-C transcripts enzymatically amplified and sequenced using a limited number of selected oligonucleotide.
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申请公布号 |
US5593830(A) |
申请公布日期 |
1997.01.14 |
申请号 |
US19940283203 |
申请日期 |
1994.07.28 |
申请人 |
REGENTS OF THE UNIVERSITY OF MINNESOTA |
发明人 |
SANTAMARIA, PEDRO;BOYCE-JACINO, MICHAEL T.;BARBOSA, JOSE J.;RICH, STEPHEN S.;FARAS, ANTHONY J. |
分类号 |
C12Q1/68;(IPC1-7):C12Q1/68;C12P19/34;C07H21/04 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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